Traditional medicine practices among Gugu cultural practitioners in Kibuga District, Uganda have been influenced by traditional beliefs and modern healthcare systems. A mixed-methods study combining qualitative interviews with quantitative surveys to gather data from Gugu cultural practitioners, community members, and local healthcare providers. Gugu practitioners reported a significant reliance on traditional remedies for chronic conditions (45% of respondents) compared to acute illnesses (10%). Traditional medicine plays a crucial role in the overall health management of Gugu cultural practitioners, influencing both community health and healthcare integration. Integrate traditional practices into formal healthcare systems to enhance holistic health services and improve patient outcomes.