HIV/AIDS remains a significant public health issue in Ghana, particularly among female sex workers who face heightened risks due to their occupational circumstances. The longitudinal ethnographic study employed qualitative research methods, including semi-structured interviews and focus group discussions with female sex workers and healthcare providers. Participant observation was also conducted to gather contextual insights. During the first year of intervention, only 30% of participants reported actively using prevention techniques recommended by the programmes. By the third year, this figure increased to 45%, indicating a gradual but significant improvement in programme uptake over time. The findings highlight the importance of sustained engagement and tailored support for effective HIV/AIDS prevention among female sex workers in Accra. Programmes should incorporate regular follow-ups and address stigma, while healthcare providers need to provide more comprehensive education on prevention methods.