Urban youth in Lagos, Nigeria have shown varying levels of acceptance towards Covid-19 vaccines due to cultural and behavioural factors. A mixed-methods approach combining qualitative interviews with a structured questionnaire among 200 urban youth participants aged 18-35, supplemented by focus group discussions. Urban youths in Lagos presented significant concerns about vaccine efficacy and side effects (46%), while 37% were undecided due to misinformation. Cultural norms also played a role with 29% citing traditional beliefs as a deterrent. The acceptance rate of vaccines among urban youth was significantly lower than the general population, highlighting the importance of addressing cultural barriers and improving vaccine education. Develop culturally sensitive communication strategies that address misinformation and integrate community leaders into health promotion campaigns.