HIV/AIDS remains a significant public health challenge in northern Ghana, particularly among rural women farmers who are disproportionately affected by poverty and limited access to healthcare. A qualitative study employing semi-structured interviews with 50 randomly selected rural women farmers from four districts in northern Ghana. Data analysis followed thematic content analysis. The prevalence of HIV/AIDS among the sampled population was found to be 24%, with a higher proportion (36%) in younger women compared to older ones, suggesting generational differences in vulnerability. Rural women farmers in northern Ghana face significant barriers to accessing HIV prevention and treatment services. These include socio-economic constraints and cultural taboos surrounding sexual health. Integrating community-based HIV education programmes into farming cooperatives could improve awareness and access to preventive measures. Strengthening healthcare infrastructure, particularly for rural areas, is also crucial.