Mobile food safety training programmes have been implemented to improve hygiene practices among small-scale fishers in various regions globally. However, their effectiveness remains underexplored in specific contexts like Tanzania. A mixed-methods approach was employed, including pre- and post-training assessments through surveys and semi-structured interviews. Data were analysed using descriptive statistics and thematic analysis. Among the surveyed fishers, 75% reported improved hygiene practices after training, with a significant increase in awareness of food safety guidelines (p < 0.01). Mobile food safety training programmes show promise for enhancing hygiene practices among small-scale fishers in Tanzania. Further studies should investigate the long-term effects and scalability of such programmes, while policymakers could consider integrating mobile training into existing agricultural support initiatives.