Healthcare workers in Nairobi face significant stress due to demanding work environments. A mixed-methods approach was employed, including pre- and post-intervention surveys and focus groups conducted with a sample of 120 Nairobi-based healthcare workers. Participants reported a statistically significant decrease in stress levels (p < 0.05) after six months of yoga sessions, with an average reduction of 34% compared to baseline measurements. Yoga sessions appear effective in managing stress among Nairobi’s healthcare workers, warranting further investigation and potential policy implementation. Implementing regular yoga sessions as part of occupational health programmes for all healthcare workers in Nairobi.