Afro-Linguistics is a branch of linguistics that focuses on languages spoken in Africa and their classification within broader language families. In Nigeria, where over 500 indigenous languages are documented, understanding these linguistic structures has significant implications for cultural preservation, education systems, and national identity. This study employs an interdisciplinary methodology involving historical linguistics, comparative methods, and computational tools to analyse the syntactic and semantic elements of Nigerian languages. Semiotic analyses are used to interpret cultural nuances embedded in language structures. This theoretical framework not only provides a robust foundation for further linguistic research in Nigeria but also offers insights into broader African language studies. It emphasizes the importance of preserving and documenting these unique linguistic systems. Future researchers are encouraged to apply this framework in diverse contexts, including education reform and cultural heritage projects. Collaboration between linguists, anthropologists, and educators can lead to more effective language preservation strategies.