HIV/AIDS is a significant public health issue in Nairobi's Red Light Districts, where female sex workers (FSWs) are at high risk of infection due to their occupational exposure and socio-economic vulnerabilities. A mixed-methods approach will be employed, integrating qualitative interviews with FSWs, quantitative data analysis of health service utilization patterns, and secondary literature review to inform the framework development process. This theoretical framework provides a robust foundation for designing targeted interventions that can be implemented in the Nairobi Red Light Districts, potentially reducing HIV transmission rates among FSWs. Healthcare providers and policymakers should prioritise community engagement, confidentiality measures, financial support services, and policy advocacy to create an environment conducive to effective prevention efforts.