Cotton production in northern Ghana faces significant challenges from pests that threaten both yield and quality. Traditional chemical pesticides are widely used but pose environmental risks. A mixed-methods approach combining surveys with qualitative interviews was employed to assess farmer perceptions and practices related to biopesticide use. Farmers reported a preference for biopesticides over chemical pesticides, citing reduced environmental contamination as the primary driver. However, there is variability in application frequency based on pest severity (e.g., $2-4$ applications per season). Biopesticide adoption has led to enhanced sustainability but requires further optimization of application strategies. Further research should focus on developing cost-effective biopesticides and training programmes for farmers to maximise benefits. Cotton, Biopesticides, Farmers' Practices, Pest Management, Northern Ghana