HIV (Human Immunodeficiency Virus) prevention is crucial among female youth workers in Nigeria due to their significant role in community health and education. Qualitative research methods were employed, including semi-structured interviews and focus group discussions with participants from various Lagos-based organizations working with young people. Participants reported an average of 60% improvement in knowledge about HIV transmission and prevention techniques post-training. The training programme was effective in improving HIV prevention practices among female youth workers, leading to a reduction in perceived risk behaviors and better understanding of viral load management strategies. Continuation and expansion of the training programme should be considered, with recommendations for further research into long-term impact and sustainability.